CHARACTERIZATION OF THE PHYSICOCHEMICAL PROPERTIES OF THE BIOSURFACTANT PRODUCED BY L. ACIDOPHILUS AND L. PENTOSUS

Authors

  • Nagea Abdalsadiq Department of Microbiology, Faculty of Science and Technology, Universiti Sains Islam Malaysia (USIM), 71800Nilai, Negeri Sembilan, Malaysia.
  • Zaiton Hassan Department of Microbiology, Faculty of Science and Technology, Universiti Sains Islam Malaysia (USIM), 71800Nilai, Negeri Sembilan, Malaysia.
  • Mohd LANI School of Food Science and Technology, Universiti Malaysia Terengganu (UMT), 21030 Kuala Terengganu, Terengganu, Malaysia

DOI:

https://doi.org/10.53555/ephijse.v7i4.193

Keywords:

Biosurfactant, Emulsification index, Surface tension, critical micelle concentration, L. acidophilus, L. pentosus

Abstract

Biosurfactants or surface-active compounds are biodegradable, non-toxic and eco-friendly compounds released by microorganisms. Biosurfactants are amphiphilic compounds cause surface tension reduction both aqueous solutions and hydrocarbon mixtures. The main purpose of this work was to characterize biosurfactant produced by Lactobacillus strains.  Identification using 16s rDNA identified the isolates as L. acidophilus for Fm1 and L. pentosus for Y1. Effect different parameters (temperature, PH and Salinity) were studied to evaluate the stability of biosurfactant after treatment.  In addition, critical micelle concentration of biosurfactant, emulsification index and viscosity reduction of palm and engine oils have been studied. The results revealed that, the biosurfactant from L. acidophilus and L. pentosus maintains its emulsifications activities unaffected in the wide range of parameter's study except slightly decreasing in emulsification index values at salinity 15%. The maximum reduction in surface tension was 18.05 mN/m with minimum concentration of critical micelle concentration of 7.5 mg/ml and high decrease of palm and engine oil viscosity of 110.1 and 165.3% respectively. This study concluded that, the emulsification activity, the surface activity and the stability to heat treatment, different PH and salinity of biosurfactant of Lactobacillus strains revealed the application of the biosurfactant in food, pharmaceutical, cosmetics industries and oil recovery. 

References

. Cameotra, S.S., Makkar, R.S., Kaur, J, & Mehta, S.K. 2010. Synthesis of biosurfactants and their advantages to microorganisms and mankind. Adv. Exp. Med. Biol., 672:261–280.

. Mukherjee, S., Das, P., & Sen, R. 2006. Towards commercial production of microbial surfactants.Trends Bioethanol 24, 509–515.

. Banat. I. M., Franzetti.A., Gandolfi. I., Bestetti . G,m Martinotti M. G., Fracchia, L., Smyth, T.J., & Marchant, R. 2010. Applied Microbiology and Biotechnology June, 87, 2: 427 444.

. Mcinerney, M. J., Javaheri, M. & Nagle, D. P. 1990. Properties of the biosurfactant produced by Bacillus licheniformis strain JF-2. J. Ind. Microbiol., 5, 95-102.

. Conter, M., Muscariello, T., Zanardi, E., Ghidini, S., Vergara, A., Campanini, G. & Lanieri, A. 2005.

“Characterization of Lactic Acid Bacteria Isolated from an Italian Dry Fermented Sausage”. Romanian Biotechnological Letters. Vol. 14: p. 167-174.

. Yin, H., J. Qiang, Y. Jia, J. Ye, Peng, H. Qin, N. Zhang & B. He. 2009. Characteristics of biosurfactant produced by Pseudomonas aeruginosa S6 isolated from oil-containing wastewater. Process Biochemistry. Vol. 44: p. 302-308.

. Cooper, D. G & Goldenberg, B. G. 1987. Surface-Active Agents from Two Bacillus Species. Appl Environ Microbiol , 53(2): p. 224–229.

. El-Sersy, N. 2012. Plackett-Burman design to optimize biosurfactant production by marine Bacillus subtilis N10. Rom. Biotechnol. Lett. 17 (2), 7049–7064.

. Anandaraj B, Thivakaran P. 2010. Isolation and production of biosurfactant producing organism from oil spilled soil. J Biosci Tech 1: 120–126.

. Devesa-Rey R., Bustos G., Cruz, J.M. & Moldes, A.B. 2011. Optimisation of entrapped activated carbon conditions to remove coloured compounds from winery wastewaters. Bioresource Technology, 102, 6437–6442.

. Jara AM, Andrade RF, & Campos-Takaki GM. 2013. Physicochemical characterization of tensioactive produced by Geobacillus stearothermophilus isolated from petroleum-contaminated soil. Colloids Surf. B Biointerf. 101:315-318.

. Augustin, M & Hippolyte, M. T. 2012. Screening of biosurfactants properties of cell-free supernatants of cultures of Lactobacillus spp. isolated from a local fermented milk (Pendidam) of Ngaoundere (Cameroon). International Journal of Engineering Research and Applications. Vol. 2 (5): p. 974-985.

. Desai, J.D., & Banat, I.M. 1997. Microbial production of surfactant and their commercial potential. Microbiology and Molecular Biology Reviews. Vol. 61: p.47–64, 1997.

. Eduardo J., Gudina, J., José A., Teixeira & Lígia R. Rodrigues. 2011. Biosurfactant-Producing Lactobacilli: Screening, Production Profiles, and Effect of Medium Composition. Applied and Environmental Soil Science. Article ID 201254.

. Khopade, A., Biao, R., Liu, X., Mahadik, K., Zhang, L & Kokare, C. 2012. Production and stability studies of the biosurfactant isolated from marine Nocardiopsis sp. B4. Desalination 3, 198–204.

. Augustin, M., Hippolyte, M.T & Ra€ıssa, K.R., 2013. Antibacterial activity of Lactobacillus’ biosurfactants against Pseudomonas spp. isolated from fresh beef. Novus Int. J. Biotechnol. Biosci., 2, 7–22.

. Gudina, E. J., Teixeira, J.A. & Rodrigues, L. R. (2010). Isolation and functional characterization of a biosurfactant produced by Lactobacillus paracasei. Colloids and Surfaces B: Biointerfaces, 76:298-304.

. Augustin, M., Majesté, P. M., Hippolyte, M.T & Léopold, T. N. 2015. Effect of Biosurfactants Extracted from a Locally Fermented Milk (Pendidam) on Its Shelf Life. Journal of Advances in Biology & Biotechnology. Vol. 3(1): p. 12-22.

. Rodrigues, L., Banat, IM. Teixeira, J & Oliveira, R. 2006. Biosurfactants: potential applications in medicine. J Antimicrob Chemother. Vol. 57: p. 609–618.

. Joshi, S., Bharucha, C. & Anjana. 2008. “Production of biosurfactant and antifungal compound by fermented food isolate Bacillus subtilis 20B”. J. Desai Bioresource Technology. Vol. 99. p. 4603–4608.

. Fernandes, E.C.R et al. 2013. “Study of Biosurfactant “Cocktails” with Enhanced Properties. (Master Thesis)”. Minho's University, Portuguese. pp. 3.

. Rodrigues, L., Teixeira, J., Oliveira, R. & Van der Mei, H. 2005. “Response surface optimization of the medium components for the production of biosurfactants by probiotic bacteria”. Process Biochemistry. Vol. 41: p.1-10.

. Fracchia L, Cavallo M, Allegrone G & Martinotti MG. 2010. A Lactobacillus-derived biosurfactant inhibits biofilm formation of human pathogenic Candida albicans biofilm producers. Appl Microbiol Biotechnol 2:827–837.

. Batista, S.B., A.H. Mounteer, F.R. Amorim, & M.R. Totola. 2006. Isolation and characterization of biosurfactant/bioemulsifier-producing bacteria from petroleum contaminated sites. Bioresource Technology. Vol. 97: p. 868-875.

. Siripun, S. 2011. Isolation of Biosurfactant–Producing Bacteria with Antimicrobial Activity against Bacterial Pathogens. Environment Asia. Vol. 4 (1): p. 1-5.

. Bento, M., Camargo, F. D. O., Okeke, BC & Frankenberger WT Jr. 2005. Diversity of biosurfactant producing microorganisms isolated from soils contaminated with diesel oil. Microbiol Res. Vol. 160 (3): p. 249-255.

. Satpute, S.K., Banat, I.M., Dhakephalkar, P.K., Banpurkar, A.G., et al., 2010. Biosurfactants, bioemulsifiers and exopolysaccharides from marine microorganisms. Biotechnol. Adv., 28, 436– 450.

. Cooper, D. G., C. R. MacDonald, S. J. B. Duff, & N. Kosaric. 1981. Enhanced production of surfactin from Bacillus slubtilis by continuous product removal and metal cation additions. Appl. Environ. Microbiol., 42:408-412, 1981.

. Nadielly R. Andrade Silva, Marcos A. C. Luna, André L. C. M. A. Santiago, Luciana O. Franco, Grayce K. B. Silva, Patrícia M. de Souza, Kaoru Okada, Clarissa D. C. Albuquerque, Carlos A. Alves da Silva, andGalba M. Campos-Takaki. 2014. Biosurfactant-and-Bioemulsifier Produced by a Promising Cunninghamella echinulata Isolated from Caatinga Soil in the Northeast of Brazil. Campos-Takaki Int. J. Mol. Sci. 2014, 15, 15377-15395.

. Sen, R. & Swaminathan, T. 2005. “Characterization of concentration and purification parameters and operating conditions for the small-scale recovery of surfactin, Process”. Biochem. Vol. 40: p. 2953–2958.

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Published

2021-12-27

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Vol. 7 No. 4 (2021): EPH - International Journal of Science And Engineering (ISSN: 2454 - 2016)

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